CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells.Flow cytometric, Western blot, and deglycosylation analyses showed surface expression of a glycosylated CLEC9A dimer of 40 and 45 kD under reducing conditions. The deglycosylated dimer was approximately 30 and 35 kD. RT-PCR detected CLEC9A expression in most human tissues examined, with highest levels in brain, thymus, and spleen.