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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using HLA-DRA mouse mAb against Ramos (1), and Raji (2) cell lysate.
Immunofluorescence analysis of Hela cells using HLA-DRA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of MCF-7 cells using HLA-DRA mouse mAb (green) and negative control (red).

Mouse Monoclonal Antibody to HLA-DRA

  • 货号:

    31113
  • 别名:

    MLRW; HLA-DRA1
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IF,FCM
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P01903
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to HLA-DRA

Description

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.

References

1.Int J Immunogenet. 2014 Dec;41(6):508-11.
2.Am J Rhinol Allergy. 2012 Jan-Feb;26(1):12-7.

Specification

Aliases MLRW; HLA-DRA1
Entrez GeneID 3122
Swissprot P01903
clone 1C11A5
WB Predicted band size 28.6kDa
Host/Isotype Mouse IgG2b
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of human HLA-DRA (AA: 26-254) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IF 1/100 - 1/500
FCM 1/200 - 1/400
ELISA 1/10000
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