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Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Mouse muscle tissue and Hepg2 cell, Primary antibody: P07223(RAF1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using P07223(RAF1 Antibody) at dilution 1/40. (Original magnification: ×200)

Rabbit Polyclonal Antibody to RAF1

  • 货号:

    P07223
  • 别名:

    NS5; CRAF; Raf-1; c-Raf; CMD1NN
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC
  • 反应种属:

    Human, Mouse, Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P04049
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to RAF1

Description

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Specification

Aliases NS5; CRAF; Raf-1; c-Raf; CMD1NN
Swissprot P04049
WB Predicted band size 73 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen Fusion protein of human RAF1
Formulation Purified antibody in PBS with 0.05% sodium azide and 50% glycerol.

Application

WB 1/500-1/2000
IHC 1/25-1/100
ELISA 1/5000-1/10000
XML 地图