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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Western blot analysis using TOP2A mouse mAb against PC-12 (1), Hela (2), Jurkat (3), and K562 (4) cell lysate.
Immunofluorescence analysis of Hela cells using TOP2A mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis of Hela cells using TOP2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using TOP2A mouse mAb (green) and negative control (red).

Mouse Monoclonal Antibody to TOP2A

  • 货号:

    31853
  • 别名:

    TOP2; TP2A
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IF,FCM
  • 反应种属:

    Human, Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P11388
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to TOP2A

Description

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

References

1.Asian Pac J Cancer Prev. 2018 Dec 25;19(12):3581-3589.
2.BMC Cancer. 2018 Mar 27;18(1):331.

Specification

Aliases TOP2; TP2A
Entrez GeneID 7153
Swissprot P11388
clone 6C12G12
WB Predicted band size 174.4kDa
Host/Isotype Mouse IgG2b
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Rat
Immunogen Purified recombinant fragment of human TOP2A (AA: 1100-1530) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IF 1/100 - 1/500
FCM 1/200-1/400
ELISA 1/10000
XML 地图