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Western blot analysis of extract from HeLa cells, using NF-κB p105/p50 (phospho-Ser337) antibody (P40017, Lane 1 and 2).
Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using NFκB-p105/p50(Phospho-Ser337) Antibody P40017.
Immunofluorescence staining of methanol-fixed HeLa cells using NF-κB p105/p50 (phospho-Ser337) antibody (P40017, Red).
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NF-κB p105/p50 (phospho-Ser337) antibody (P40017).

Rabbit Polyclonal Antibody to NFkB-p105/p50(Phospho-Ser337)

  • 货号:

    P40017
  • 别名:

    p50; KBF1; NF-kB1; NFKB-p50; NFkappaB
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P19838
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Polyclonal Antibody to NFkB-p105/p50(Phospho-Ser337)

Description

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.

References

Beg A.A., Baldwin A.S. Jr.Oncogene 9:1487-1492(1994) Guizani-Tabbane L., Ben-Aissa K., Belghith M., Sassi A., Dellagi K.Infect. Immun. 72:2582-2589(2004) Beinke S., Robinson M.J., Hugunin M., Ley S.C.Mol. Cell. Biol. 24:9658-9667(2004)

Specification

Aliases p50; KBF1; NF-kB1; NFKB-p50; NFkappaB
Entrez GeneID 4790;
Swissprot P19838
WB Predicted band size 50,120kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen Peptide sequence around phosphorylation site of serine 337(R-K-S(p)-D-L) derived from Human NFkB-p105/p50.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500-1/1000
IHC 1/50-1/100
IF 1/100-1/200
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