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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using MSH2 mouse mAb against MCF-7 (1), A431 (2),K562 (3),Hela (4),Raji (5),A549 (6),NIH/3T3 (7),cos-7 (8), and Hek293-e6 (9) cell lysate.
Immunofluorescence analysis of Hela cells using MSH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using MSH2 mouse mAb (green) and negative control (red).
Flow cytometric analysis of HepG2 cells using MSH2 mouse mAb (green) and negative control (red).
Mouse Monoclonal Antibody to MSH2
-
货号：
32046 -
别名：
FCC1; COCA1; HNPCC; LCFS2; hMSH2; HNPCC1 -
应用：
WB,IF,FCM -
反应种属：
Human, Mouse, Monkey -
抗体类型：
Primary antibody -
Swissprot：
P43246 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to MSH2
Description |
|---|
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. |
References |
|---|
| 1,Genes Chromosomes Cancer. 2020 Feb;59(2):111-118. 2,Clin Epigenetics. 2019 Oct 30;11(1):153. |
Specification |
|
|---|---|
| Aliases | FCC1; COCA1; HNPCC; LCFS2; hMSH2; HNPCC1 |
| Entrez GeneID | 4436 |
| Swissprot | P43246 |
| clone | 4D1G12 |
| WB Predicted band size | 104.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Monkey |
| Immunogen | Purified recombinant fragment of human MSH2 (AA: 2-151) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Western blot analysis using MSH2 mouse mAb against MCF-7 (1), A431 (2),K562 (3),Hela (4),Raji (5),A549 (6),NIH/3T3 (7),cos-7 (8), and Hek293-e6 (9) cell lysate.
- Immunofluorescence analysis of Hela cells using MSH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of Hela cells using MSH2 mouse mAb (green) and negative control (red).
- Flow cytometric analysis of HepG2 cells using MSH2 mouse mAb (green) and negative control (red).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#32046
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