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Western blot analysis of PKC iota/zeta (phospho-Thr412/410) expression in MCF7 TNFa-treated, NIH3T3 LPS-treated, PC12 UV-treated whole cell lysates.
Immunofluorescent analysis of PKC iota/zeta (phospho-Thr412/410) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunohistochemical analysis of PKC iota/zeta (phospho-Thr412/410) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Polyclonal Antibody to PKC iota/zeta (phospho-Thr412/410)
-
货号：
P41218 -
别名：
DXS1179E; Protein kinase C iota type; Atypical protein kinase C-lambda/iota; PRKC-lambda/iota; aPKC-lambda/iota; nPKC-iota; PKC2; Protein kinase C zeta type; nPKC-zeta -
应用：
WB,IHC,IF -
反应种属：
Human,Mouse,Rat -
抗体类型：
Primary antibody -
Swissprot：
P41743 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Polyclonal Antibody to PKC iota/zeta (phospho-Thr412/410)
Specification |
|
|---|---|
| Aliases | DXS1179E; Protein kinase C iota type; Atypical protein kinase C-lambda/iota; PRKC-lambda/iota; aPKC-lambda/iota; nPKC-iota; PKC2; Protein kinase C zeta type; nPKC-zeta |
| Entrez GeneID | 5584; |
| Swissprot | P41743 |
| WB Predicted band size | 78kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PKC iota/zeta. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| IF | 1/100-1/300 |
Product Image
- Western blot analysis of PKC iota/zeta (phospho-Thr412/410) expression in MCF7 TNFa-treated, NIH3T3 LPS-treated, PC12 UV-treated whole cell lysates.
- Immunofluorescent analysis of PKC iota/zeta (phospho-Thr412/410) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
- Immunohistochemical analysis of PKC iota/zeta (phospho-Thr412/410) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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