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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using VCP mouse mAb against Hela (1), A549 (2), NIH/3T3 (3), Raw264.7 (4), SH-SY5Y (5), MCF-7 (6), and A431 (7) cell lysate.
Immunofluorescence analysis of Hela cells using VCP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of MOLT4 cells using VCP mouse mAb (green) and negative control (red).
Flow cytometric analysis of HL-60 cells using VCP mouse mAb (green) and negative control (red).
Flow cytometric analysis of THP-1 cells using VCP mouse mAb (green) and negative control (red).
Mouse Monoclonal Antibody to VCP
-
货号：
32180 -
别名：
p97; TERA; CDC48; FTDALS6 -
应用：
WB,IF,FCM -
反应种属：
Human, Mouse -
抗体类型：
Primary antibody -
Swissprot：
P55072 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to VCP
Description |
|---|
This protein forms a homohexameric complex that interacts with a variety of cofactors and extracts ubiquitinated proteins from lipid membranes or protein complexes. Mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Charcot-Marie-Tooth disease in human patients. |
References |
|---|
| 1,mBio. 2020 Apr 14;11(2):e00467-20. 2,Nat Commun. 2021 Jan 29;12(1):713. |
Specification |
|
|---|---|
| Aliases | p97; TERA; CDC48; FTDALS6 |
| Entrez GeneID | 7415 |
| Swissprot | P55072 |
| clone | 8E4A4 |
| WB Predicted band size | 89.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Purified recombinant fragment of human VCP (AA:707-806) expressed in Mammalian system |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Western blot analysis using VCP mouse mAb against Hela (1), A549 (2), NIH/3T3 (3), Raw264.7 (4), SH-SY5Y (5), MCF-7 (6), and A431 (7) cell lysate.
- Immunofluorescence analysis of Hela cells using VCP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of MOLT4 cells using VCP mouse mAb (green) and negative control (red).
- Flow cytometric analysis of HL-60 cells using VCP mouse mAb (green) and negative control (red).
- Flow cytometric analysis of THP-1 cells using VCP mouse mAb (green) and negative control (red).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#32180
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