Description
MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 . Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 . Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 .

References
Haupt, Y. et al. (1997) Nature 387, 296-299. Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603. Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981. Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.

Specification
Aliases	HDMX; hdm2;
Entrez GeneID	4193;
Swissprot	Q00987
WB Predicted band size	90kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Peptide sequence around aa.18~22(Q-I-P-A-S) derived from Human MDM2.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

Application
WB	1/500-1/1000