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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using RHOA mouse mAb against Jurlat (1), MCF-7 (2),A431 (3), and Hela (4) cell lysate.

Immunofluorescence analysis of Hela cells using RHOA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using RHOA mouse mAb (green) and negative control (red).

Flow cytometric analysis of HepG2 cells using RHOA mouse mAb (green) and negative control (red).

Flow cytometric analysis of Jurkat cells using RHOA mouse mAb (green) and negative control (red).

Flow cytometric analysis of K562 cells using RHOA mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissues using RHOA mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissues using RHOA mouse mAb with DAB staining.

Mouse Monoclonal Antibody to RHOA

货号：
32237
别名：
ARHA; ARH12; RHO12; EDFAOB; RHOH12
产品上架时间：
2024-01-01
应用：
WB,IHC,IF,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P61586
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥2180

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Mouse Monoclonal Antibody to RHOA

Description
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Description

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

References
1.Immunity.2020 Jul 14;53(1):187-203.e8. 2.Nat Commun.2021 Apr 13;12(1):2226.

Specification
Aliases	ARHA; ARH12; RHO12; EDFAOB; RHOH12
Entrez GeneID	387
Swissprot	P61586
clone	7E7H4
WB Predicted band size	22kDa
Host/Isotype	Mouse IgG2b
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of human RHOA (AA: 1-190) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide