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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using NEDD9 mouse mAb against MCF-7 (1), Hela (2), C2C12 (3),and Hek293 (4) cell lysate.
Immunofluorescence analysis of Hela cells using NEDD9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of HepG2 cells using NEDD9 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Jurkat cells using NEDD9 mouse mAb (green) and negative control (red).
Flow cytometric analysis of K562 cells using NEDD9 mouse mAb (green) and negative control (red).

Mouse Monoclonal Antibody to NEDD9

  • 货号:

    32248
  • 别名:

    CAS2; CASL; HEF1; CAS-L; CASS2
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IF,FCM
  • 反应种属:

    Human, Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q14511
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to NEDD9

Description

The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

References

1.Biomolecules.2018 Dec 10;8(4):169.
2.Biochem Biophys Res Commun.2019 Jan 29;509(1):201-208.

Specification

Aliases CAS2; CASL; HEF1; CAS-L; CASS2
Entrez GeneID 4739
Swissprot Q14511
clone 3F7A9
WB Predicted band size 92.9kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Rat
Immunogen Purified recombinant fragment of human NEDD9 (AA: 82-398) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IF 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
XML 地图