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Mouse Monoclonal Antibody to NEDD9
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货号:
32248 -
别名:
CAS2; CASL; HEF1; CAS-L; CASS2 -
应用:
WB,IF,FCM -
反应种属:
Human, Rat -
抗体类型:
Primary antibody -
Swissprot:
Q14511 -
规格:
-
数量:
-+ -
说明书:
目录价¥2180

Mouse Monoclonal Antibody to NEDD9
Description |
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The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
References |
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1.Biomolecules.2018 Dec 10;8(4):169. 2.Biochem Biophys Res Commun.2019 Jan 29;509(1):201-208. |
Specification |
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Aliases | CAS2; CASL; HEF1; CAS-L; CASS2 |
Entrez GeneID | 4739 |
Swissprot | Q14511 |
clone | 3F7A9 |
WB Predicted band size | 92.9kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Rat |
Immunogen | Purified recombinant fragment of human NEDD9 (AA: 82-398) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
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WB | 1/500 - 1/2000 |
IF | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Western blot analysis using NEDD9 mouse mAb against MCF-7 (1), Hela (2), C2C12 (3),and Hek293 (4) cell lysate.
- Immunofluorescence analysis of Hela cells using NEDD9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of HepG2 cells using NEDD9 mouse mAb (green) and negative control (red).
- Flow cytometric analysis of Jurkat cells using NEDD9 mouse mAb (green) and negative control (red).
- Flow cytometric analysis of K562 cells using NEDD9 mouse mAb (green) and negative control (red).
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#32248

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