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Western blot analysis using TOP1 mouse mAb against HUVEC (1), Jukat (2),MCF-7 (3),PC-12 (4),Hela (5),NIH/3T3 (6) and HCT116 (7) cell lysate.
Immunofluorescence analysis of Hela cells using TOP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using TOP1 mouse mAb (green) and negative control (red).
Mouse Monoclonal Antibody to TOP1
-
货号：
32389 -
别名：
TOPI -
应用：
WB,IF,FCM -
反应种属：
Human, Mouse -
抗体类型：
Primary antibody -
Swissprot：
P11387 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to TOP1
Description |
|---|
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. |
References |
|---|
| 1.Cell. 2021 May 13;184(10):2618-2632.e17. 2.Nat Commun. 2020 Aug 7;11(1):3940. |
Specification |
|
|---|---|
| Aliases | TOPI |
| Entrez GeneID | 7150 |
| Swissprot | P11387 |
| clone | 3D4B1 |
| WB Predicted band size | 90.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Purified recombinant fragment of human TOP1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IF | 1/100 - 1/400 |
| FCM | 1/200 - 1/400 |
Product Image
- Western blot analysis using TOP1 mouse mAb against HUVEC (1), Jukat (2),MCF-7 (3),PC-12 (4),Hela (5),NIH/3T3 (6) and HCT116 (7) cell lysate.
- Immunofluorescence analysis of Hela cells using TOP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of Hela cells using TOP1 mouse mAb (green) and negative control (red).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#32389
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