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Western blot analysis of extracts from 3T3 and HL60 cells using eIF4B(Ab-422) Antibody P41545.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4B(Ab-422) Antibody P41545(left) or the same antibody preincubated with blocking peptide(right).

Rabbit Polyclonal Antibody to eIF4B(Ab-422)

  • 货号:

    P41545
  • 别名:

  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P23588
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to eIF4B(Ab-422)

Description

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

References

Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826. Duncan, R. and Hershey, J.W. (1985) J. Biol. Chem. 260, 5493-5497. Duncan, R.F. and Hershey, J.W. (1989) J. Cell Biol. 109, 1467-1481.

Specification

Entrez GeneID 1975;
Swissprot P23588
WB Predicted band size 80kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen Peptide sequence around aa.420~424 (T-G-S-E-S) derived from Human eIF4B.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500-1/1000
IHC 1/50-1/100

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#P41545

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