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Western blot analysis of lysates from A549
cells, using PKM Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (RS0002,immunoway) was used to detect the antibody.
Immunohistochemical analysis of paraffin-embedded human kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Immunohistochemical analysis of paraffin-embedded mouse lung tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Rabbit Monoclonal Antibody to PKM
-
货号：
50521 -
别名：
PKM;OIP3;PK2;PK3;PKM2;Pyruvate kinase isozymes M1/M2;Cytosolic thyroid hormone-binding protein;CTHBP;Opa-interacting protein 3;OIP-3;Pyruvate kinase 2/3;Pyruvate kinase muscle isozyme;Thyroid hormone-binding protein 1;THBP1;Tu -
应用：
WB,IHC,IF,IP,ELISA -
反应种属：
Human,Mouse,Rat, -
抗体类型：
Primary antibody -
Swissprot：
P14618 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal Antibody to PKM
Description |
|---|
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011], |
Specification |
|
|---|---|
| Aliases | PKM;OIP3;PK2;PK3;PKM2;Pyruvate kinase isozymes M1/M2;Cytosolic thyroid hormone-binding protein;CTHBP;Opa-interacting protein 3;OIP-3;Pyruvate kinase 2/3;Pyruvate kinase muscle isozyme;Thyroid hormone-binding protein 1;THBP1;Tu |
| Conjugation | Unmodified |
| Entrez GeneID | 5315 |
| Swissprot | P14618 |
| clone | 10C4A10 |
| WB Predicted band size | 57kD |
| Host/Isotype | Rabbit IgG,Kappa |
| Antibody Type | Primary antibody |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Species Reactivity | Human,Mouse,Rat, |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
Application |
|
|---|---|
| WB | 1/1000-5000 |
| IP | 1/50-200 |
| IHC | 1/200-1000 |
| ELISA | 1/5000-20000 |
Product Image
- Western blot analysis of lysates from A549 cells, using PKM Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (RS0002,immunoway) was used to detect the antibody.
- Immunohistochemical analysis of paraffin-embedded human kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
- Immunohistochemical analysis of paraffin-embedded mouse lung tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#50521
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