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Western blot analysis of lysates from A431
cells, using Lactate Dehydrogenase Isoenzyme V Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (RS0002,immunoway) was used to detect the antibody.
Immunohistochemical analysis of paraffin-embedded human appendix tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Rabbit Monoclonal Antibody to Lactate Dehydrogenase Isoenzyme V
-
货号：
50533 -
别名：
-
应用：
WB,IHC,IF,IP,ELISA -
反应种属：
Human,Mouse,Rat, -
抗体类型：
Primary antibody -
Swissprot：
P00338 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal Antibody to Lactate Dehydrogenase Isoenzyme V
Description |
|---|
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008], |
Specification |
|
|---|---|
| Conjugation | Unmodified |
| Entrez GeneID | 3939 |
| Swissprot | P00338 |
| clone | 8A4F7 |
| WB Predicted band size | 36kD |
| Host/Isotype | Rabbit IgG,Kappa |
| Antibody Type | Primary antibody |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Species Reactivity | Human,Mouse,Rat, |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
Application |
|
|---|---|
| WB | 1/500-2000 |
| IP | 1/50-200 |
| IHC | 1/200-1000 |
| ELISA | 1/5000-20000 |
Product Image
- Western blot analysis of lysates from A431 cells, using Lactate Dehydrogenase Isoenzyme V Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (RS0002,immunoway) was used to detect the antibody.
- Immunohistochemical analysis of paraffin-embedded human appendix tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
- Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
- Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#50533
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