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Western blot analysis of lysates from HaCat
cells, using Cytokeratin 5 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (RS0002,immunoway) was used to detect the antibody.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Immunohistochemical analysis of paraffin-embedded mouse lung tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Rabbit Monoclonal Antibody to Cytokeratin 5
-
货号：
50590 -
别名：
KRT5;Keratin;type II cytoskeletal 5;58 kDa cytokeratin;Cytokeratin-5;CK-5;Keratin-5;K5;Type-II keratin Kb5 -
应用：
WB,IHC,IF,IP,ELISA -
反应种属：
Human,Mouse,Rat, -
抗体类型：
Primary antibody -
Swissprot：
P13647 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal Antibody to Cytokeratin 5
Description |
|---|
keratin 5(KRT5) Homo sapiens The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008], |
Specification |
|
|---|---|
| Aliases | KRT5;Keratin;type II cytoskeletal 5;58 kDa cytokeratin;Cytokeratin-5;CK-5;Keratin-5;K5;Type-II keratin Kb5 |
| Conjugation | Unmodified |
| Entrez GeneID | 3852 |
| Swissprot | P13647 |
| clone | 2D10H3 |
| WB Predicted band size | 62kD |
| Host/Isotype | Rabbit IgG,Kappa |
| Antibody Type | Primary antibody |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Species Reactivity | Human,Mouse,Rat, |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
Application |
|
|---|---|
| WB | 1/1000-1/5000 |
| IP | 1/50-1/200 |
| IHC | 1/200-1/1000 |
| ELISA | 1/5000-1/20000 |
Product Image
- Western blot analysis of lysates from HaCat cells, using Cytokeratin 5 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (RS0002,immunoway) was used to detect the antibody.
- Immunohistochemical analysis of paraffin-embedded human tonsil tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
- Immunohistochemical analysis of paraffin-embedded mouse lung tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
- Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#50590
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