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Western blot analysis of lysates from A549
cells, using Prohibitin Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (RS0002,immunoway) was used to detect the antibody.
Immunohistochemical analysis of paraffin-embedded human kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Rabbit Monoclonal Antibody to Prohibitin
-
货号：
50604 -
别名：
PHB;Prohibitin -
应用：
WB,IHC,IF,IP,ELISA -
反应种属：
Human,Mouse,Rat, -
抗体类型：
Primary antibody -
Swissprot：
P35232 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal Antibody to Prohibitin
Description |
|---|
prohibitin(PHB) Homo sapiens This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013], |
Specification |
|
|---|---|
| Aliases | PHB;Prohibitin |
| Conjugation | Unmodified |
| Entrez GeneID | 5245 |
| Swissprot | P35232 |
| clone | 5B4F10 |
| WB Predicted band size | 30kD |
| Host/Isotype | Rabbit IgG,Kappa |
| Antibody Type | Primary antibody |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Species Reactivity | Human,Mouse,Rat, |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
Application |
|
|---|---|
| WB | 1/1000-5000 |
| IP | 1/50-200 |
| IHC | 1/200-1000 |
| ELISA | 1/5000-20000 |
Product Image
- Western blot analysis of lysates from A549 cells, using Prohibitin Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (RS0002,immunoway) was used to detect the antibody.
- Immunohistochemical analysis of paraffin-embedded human kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
- Immunohistochemical analysis of paraffin-embedded mouse kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
- Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#50604
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