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Western blot analysis of lysates from HEK293 cells, using Pyruvate Dehydrogenase E1 α Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (RS0002,immunoway) was used to detect the antibody.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Rabbit Monoclonal Antibody to Pyruvate Dehydrogenase E1 α

货号：
50663
别名：
PDHA1;PHE1A;Pyruvate dehydrogenase E1 component subunit alpha;somatic form, mitochondrial;PDHE1-A type I
产品上架时间：
2024-01-01
应用：
WB,IHC,IF,IP,ELISA
反应种属：
Human,Mouse,Rat,
抗体类型：
Primary antibody
Swissprot：
P08559
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥2180

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Rabbit Monoclonal Antibody to Pyruvate Dehydrogenase E1 α

Description
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010],

Description

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010],

Specification
Aliases	PDHA1;PHE1A;Pyruvate dehydrogenase E1 component subunit alpha;somatic form, mitochondrial;PDHE1-A type I
Conjugation	Unmodified
Entrez GeneID	5160
Swissprot	P08559
clone	8E1B8
WB Predicted band size	43kD
Host/Isotype	Rabbit IgG,Kappa
Antibody Type	Primary antibody
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Species Reactivity	Human,Mouse,Rat,
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA