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A549 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Rabbit Monoclonal Antibody to Rpb1

  • 货号:

    50816
  • 别名:

    POLR2A;POLR2;DNA-directed RNA polymerase II subunit RPB1;RNA polymerase II subunit B1;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF,IP,ELISA
  • 反应种属:

    Human,Mouse,Rat,
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P24928
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Monoclonal Antibody to Rpb1

Description

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008],

Specification

Aliases POLR2A;POLR2;DNA-directed RNA polymerase II subunit RPB1;RNA polymerase II subunit B1;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1
Conjugation Unmodified
Entrez GeneID 5430
Swissprot P24928
clone 4G1H7
WB Predicted band size 192kD
Host/Isotype Rabbit IgG,Kappa
Antibody Type Primary antibody
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Species Reactivity Human,Mouse,Rat,
Formulation PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Application

WB 1/2000-1/10000
IP 1/50-1/200
IHC 1/50-1/200
ELISA 1/5000-1/20000

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#50816

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