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NIH-3T3 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Rabbit Monoclonal Antibody to NF-κB p65 (Phospho Ser536)

  • 货号:

    50861
  • 别名:

    RELA;NFKB3;Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IF,IP,ELISA
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q04206
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Monoclonal Antibody to NF-κB p65 (Phospho Ser536)

Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

Specification

Aliases RELA;NFKB3;Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Conjugation Phospho
Entrez GeneID 5970
Swissprot Q04206
clone 10G8A10
WB Predicted band size 60kD
Host/Isotype Rabbit IgG,Kappa
Antibody Type Primary antibody
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Species Reactivity Human,Mouse,Rat
Formulation PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Application

WB 1/2000-1/10000
IP 1/50-1/200
ELISA 1/5000-1/20000

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#50861

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