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Immunofluorescence analysis of NIH/3T3 cells using BCL-2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of 3T3L1 cells using BCL-2 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues (left) and human brain tissues (right) using BCL-2 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to BCL-2
-
货号：
30077 -
别名：
Bcl-2; BCL2 -
应用：
IHC,IF,FCM -
反应种属：
Human,Mouse -
抗体类型：
Primary antibody -
Swissprot：
P10415 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to BCL-2
Description |
|---|
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.Tissue specificity: Expressed in a variety of tissues. |
References |
|---|
| 1. J Biol Chem. 2010 Mar 26;285(13):9770-9. 2. Pharmacogenomics J. 2010 Feb 16. 3. J Int Med Res. 2009 Nov-Dec;37(6):1868-76. |
Specification |
|
|---|---|
| Aliases | Bcl-2; BCL2 |
| Entrez GeneID | 596 |
| Swissprot | P10415 |
| clone | 8E12 |
| WB Predicted band size | 26kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthetic peptide corresponding to residues surrounding BCL-2, conjugated to KLH. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
Application |
|
|---|---|
| IHC | 1/200 - 1/1000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Immunofluorescence analysis of NIH/3T3 cells using BCL-2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow cytometric analysis of 3T3L1 cells using BCL-2 mouse mAb (green) and negative control (purple).
- Immunohistochemical analysis of paraffin-embedded human colon cancer tissues (left) and human brain tissues (right) using BCL-2 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30077
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