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Western blot analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.
Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of Lovo cells using PDK1 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and brain tissues (right) using PDK1 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to PDK1
-
货号：
30154 -
别名：
PDK1 -
应用：
WB,IHC,IF,FCM -
反应种属：
Human,Rat,Monkey -
抗体类型：
Primary antibody -
Swissprot：
Q15118 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to PDK1
Description |
|---|
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. (provided by RefSeq). Tissue specificity: Expressed predominantly in the heart. |
References |
|---|
| 1. Nat Cell Biol. 2008 Feb;10(2):127-37. 2. Blood. 2008 Apr 1;111(7):3723-34. 3. J Biol Chem. 2007 Apr 20;282(16):12272-89. |
Specification |
|
|---|---|
| Aliases | PDK1 |
| Entrez GeneID | 5163 |
| Swissprot | Q15118 |
| clone | 4A11 |
| WB Predicted band size | 44kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human PDK1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Western blot analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.
- Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow cytometric analysis of Lovo cells using PDK1 mouse mAb (green) and negative control (purple).
- Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and brain tissues (right) using PDK1 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30154
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