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Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Western blot analysis using PRKAA1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.

Western blot analysis using PRKAA1 mouse mAb against COS7(1),C2C12(2),NIH/3T3(3) cell lysate.

Immunofluorescence analysis of NTERA-2 cells using PRKAA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of PC-2 cells using PRKAA1 mouse mAb (green) and negative control (purple).

Immunohistochemical analysis of paraffin-embedded human ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse mAb with DAB staining.

Mouse Monoclonal Antibody to PRKAA1

货号：
30172
别名：
AMPK; AMPKa1; MGC33776; MGC57364; PRKAA1
产品上架时间：
2024-01-01
应用：
WB,IHC,IF,FCM
反应种属：
Human,Mouse,Rat,Monkey
抗体类型：
Primary antibody
Swissprot：
Q13131
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥2180

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Mouse Monoclonal Antibody to PRKAA1

Description
The protein belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Description

The protein belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

References
1. Oncol Rep. 2008 Dec;20(6):1553-9. 2. Placenta. 2008 Dec;29(12):1003-8.

Specification
Aliases	AMPK; AMPKa1; MGC33776; MGC57364; PRKAA1
Entrez GeneID	5562
Swissprot	Q13131
clone	2B7
WB Predicted band size	64kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat,Monkey
Immunogen	Purified recombinant fragment of human PRKAA1 expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide.