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Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Immunofluorescence analysis of Hela cells using MAP3K5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of Hela cells using MAP3K5 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and prostate tissues (right) using MAP3K5 mouse mAb with DAB staining.

Mouse Monoclonal Antibody to MAP3K5

  • 货号:

    30225
  • 别名:

    ASK1; MEKK5; MAPKKK5
  • 产品上架时间:

    2024-01-01
  • 应用:

    IHC,IF,FCM
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q99683
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to MAP3K5

Description

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.

References

1. Mol Cancer. 2009 Dec 27;8:130.
2. J Biol Chem. 2009 Oct 30;284(44):30372-82.

Specification

Aliases ASK1; MEKK5; MAPKKK5
Entrez GeneID 4217
Swissprot Q99683
clone 2E4
WB Predicted band size 55kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of human MAP3K5 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

IHC 1/200 - 1/1000
IF 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
XML 地图