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Mouse Monoclonal Antibody to CASP8
-
货号:
30279 -
别名:
CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8; FLJ17672; MGC78473 -
应用:
WB,IHC,FCM -
反应种属:
Human,Mouse,Monkey,Rat -
抗体类型:
Primary antibody -
Swissprot:
Q14790 -
规格:
-
数量:
-+ -
说明书:
目录价¥2180

Mouse Monoclonal Antibody to CASP8
Description |
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This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. |
References |
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1. Cancer Lett. 2009 Aug 28;281(2):128-33. 2. Cell Res. 2009 Mar;19(3):358-69. |
Specification |
|
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Aliases | CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8; FLJ17672; MGC78473 |
Entrez GeneID | 841 |
Swissprot | Q14790 |
clone | 1H11 |
WB Predicted band size | 26kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Monkey,Rat |
Immunogen | Purified recombinant fragment of human CASP8 expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
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WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
- Western blot analysis using CASP8 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), NIH/3T3 (4), Cos7 (5) and PC-12 (6) cell lysate.
- Flow cytometric analysis of NIH/3T3 cells using CASP8 mouse mAb (blue) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using CASP8 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using CASP8 mouse mAb with DAB staining.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30279

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