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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Immunofluorescence analysis of Hela cells using BMI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of NIH/3T3 cells using BMI1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using BMI1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using BMI1 mouse mAb with DAB staining.

Mouse Monoclonal Antibody to BMI1

  • 货号:

    30285
  • 别名:

    PCGF4; RNF51; MGC12685; FLVI2/BMI1
  • 产品上架时间:

    2024-01-01
  • 应用:

    IHC,IF,FCM
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P35226
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to BMI1

Description

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

References

1. Mol Cancer. 2009 Nov 10;8:98.
2. Cancer Res. 2009 Dec 1;69(23):9090-5.

Specification

Aliases PCGF4; RNF51; MGC12685; FLVI2/BMI1
Entrez GeneID 648
Swissprot P35226
clone 3E3
WB Predicted band size 37kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of human BMI1 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

IHC 1/200 - 1/1000
IF 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
XML 地图