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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using NQO1 mouse mAb against A549 (1), Hela (2), MCF-7 (3) and HepG2 (4) cell lysate.
Immunofluorescence analysis of COS7 cells using NQO1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis of NIH3T3 cells using NQO1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis of C6 cells using NQO1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow cytometric analysis of HepG2 cells using NQO1 mouse mAb (green) and negative control (red).
Flow cytometric analysis of COS7 cells using NQO1 mouse mAb (green) and negative control (red).

Mouse Monoclonal Antibody to NQO1

  • 货号:

    30293
  • 别名:

    DTD; QR1; DHQU; DIA4; NMOR1; NMORI
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IF,FCM
  • 反应种属:

    Human,Rat,Monkey
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P15559
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to NQO1

Description

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

References

1. Mol Cancer Ther. 2009 Dec;8(12):3369-78.
2. J Biol Chem. 2009 Nov 27;284(48):33233-41.

Specification

Aliases DTD; QR1; DHQU; DIA4; NMOR1; NMORI
Entrez GeneID 1728
Swissprot P15559
clone 1A11
WB Predicted band size 31kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Rat,Monkey
Immunogen Purified recombinant fragment of human NQO1 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.

Application

WB 1/500 - 1/2000
IF 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
XML 地图