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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using PBK mouse mAb against A431 (1) cell lysate.
Immunofluorescence analysis of Hela cells using PBK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of Hela cells using PBK mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using PBK mouse mAb with DAB staining.
Figure5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using PBK mouse mAb with DAB staining.
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Mouse Monoclonal Antibody to PBK
-
货号：
30329 -
别名：
SPK; CT84; TOPK; Nori-3 -
应用：
WB,IHC,IF,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q96KB5 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to PBK
Description |
|---|
This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. |
References |
|---|
| Cancer Res. 2007 Jun 1;67(11):5186-94 Cancer Sci. 2010 Feb;101(2):403-11 |
Specification |
|
|---|---|
| Aliases | SPK; CT84; TOPK; Nori-3 |
| Entrez GeneID | 55872 |
| Swissprot | Q96KB5 |
| clone | 2C8 |
| WB Predicted band size | 36kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PBK expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using PBK mouse mAb against A431 (1) cell lysate.
- Immunofluorescence analysis of Hela cells using PBK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow cytometric analysis of Hela cells using PBK mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using PBK mouse mAb with DAB staining.
- Figure5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using PBK mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30329
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