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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using CK5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.
Western blot analysis using CK5 mouse mAb against PC-12(1)C2C12(2)C6(3)L1210(4)CHO3D10(5)cell lysate.
Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence analysis of HeLa cells using CK5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of HeLa cells using CK5 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using CK5 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using CK5 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Mouse colon(A) Mouse kidney(B) using CK5 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat colon(A) Rat kidney(B) using CK5 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit small intestine(A) Rabbit kidney(B) using CK5 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to CK5
-
货号：
30349 -
别名：
KRT5; K5; DDD; EBS2; KRT5A -
应用：
WB,IHC,IF,FCM -
反应种属：
Human,Mouse,Rat,Rabbit,Monkey -
抗体类型：
Primary antibody -
Swissprot：
P13647 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to CK5
Description |
|---|
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. |
References |
|---|
| Am J Surg Pathol. 2009 Nov;33(11):1615-23. J Dermatol. 2009 Aug;36(8):447-52. |
Specification |
|
|---|---|
| Aliases | KRT5; K5; DDD; EBS2; KRT5A |
| Entrez GeneID | 3852 |
| Swissprot | P13647 |
| clone | 2C2 |
| WB Predicted band size | 62.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Rabbit,Monkey |
| Immunogen | Purified recombinant fragment of human CK5 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/100 - 1/500 |
| IF | 1/50 - 1/500 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using CK5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.
- Western blot analysis using CK5 mouse mAb against PC-12(1)C2C12(2)C6(3)L1210(4)CHO3D10(5)cell lysate.
- Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Immunofluorescence analysis of HeLa cells using CK5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow cytometric analysis of HeLa cells using CK5 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using CK5 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using CK5 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded Mouse colon(A) Mouse kidney(B) using CK5 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded Rat colon(A) Rat kidney(B) using CK5 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded Rabbit small intestine(A) Rabbit kidney(B) using CK5 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30349
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