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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.
Flow cytometric analysis of Jurkat cells using NCK1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using NCK1 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to NCK1
-
货号：
30362 -
别名：
NCK; nck-1; NCKalpha -
应用：
WB,IHC,FCM -
反应种属：
Human,Monkey -
抗体类型：
Primary antibody -
Swissprot：
P16333 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to NCK1
Description |
|---|
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. |
References |
|---|
| 1. Mol Cell Biol. 2008 Mar;28(6):2035-46. 2. Cell Signal. 2010 May;22(5):848-56. |
Specification |
|
|---|---|
| Aliases | NCK; nck-1; NCKalpha |
| Entrez GeneID | 4690 |
| Swissprot | P16333 |
| clone | 5B7 |
| WB Predicted band size | 47kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | Purified recombinant fragment of human NCK1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.
- Flow cytometric analysis of Jurkat cells using NCK1 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using NCK1 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30362
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