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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using PLK1 mouse mAb against K562 (1) and Raji (2) cell lysate.
Western blot analysis using PLK1 mouse mAb against NIH/3T3(1)NRK(2)C6(3)COS-7(4)CHO3D10(5) cell lysate.
Flow cytometric analysis of NIH3T3 cells using PLK1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PLK1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using PLK1 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to PLK1
-
货号：
30396 -
别名：
PLK;STPK13 -
应用：
WB,IHC,FCM -
反应种属：
Human,Mouse,Rat,Monkey -
抗体类型：
Primary antibody -
Swissprot：
P53350 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to PLK1
Description |
|---|
Polo-like kinase 1 (Plk1) plays key roles in many aspects of mitosis. Suppression of Plk1 by p21(Waf1) is responsible for p53-dependent protection against adriamycin-induced caspase-independent mitotic death. |
References |
|---|
| 1.Cell Signal. 2011 Nov;23(11):1816-23. 2.Biochem Biophys Res Commun. 2011 Jun 24;410(1):57-61. |
Specification |
|
|---|---|
| Aliases | PLK;STPK13 |
| Entrez GeneID | 5347 |
| Swissprot | P53350 |
| clone | 3C11 |
| WB Predicted band size | 68kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human PLK1 (AA: 331-508) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/100 - 1/500 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using PLK1 mouse mAb against K562 (1) and Raji (2) cell lysate.
- Western blot analysis using PLK1 mouse mAb against NIH/3T3(1)NRK(2)C6(3)COS-7(4)CHO3D10(5) cell lysate.
- Flow cytometric analysis of NIH3T3 cells using PLK1 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PLK1 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using PLK1 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30396
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