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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using NDC80 mouse mAb against Jurkat (1) cell lysate.
Flow cytometric analysis of Hela cells using NDC80 mouse mAb (green) and negative control (red).
Flow cytometric analysis of HepG2 cells using NDC80 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Jurkat cells using NDC80 mouse mAb (green) and negative control (red).
Flow cytometric analysis of K562 cells using NDC80 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using NDC80 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using NDC80 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using NDC80 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using NDC80 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to NDC80
-
货号：
32242 -
别名：
HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80 -
应用：
WB,IHC,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
O14777 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to NDC80
Description |
|---|
This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011] |
References |
|---|
| 1.J Biol Chem.2019 Jan 11;294(2):576-592. 2.Mol Biol Cell.2020 Jul 1;31(14):1453-1473. |
Specification |
|
|---|---|
| Aliases | HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80 |
| Entrez GeneID | 10403 |
| Swissprot | O14777 |
| clone | 4B12H3 |
| WB Predicted band size | 74kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NDC80 (AA: 443-642) expressed in Mammalian. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Western blot analysis using NDC80 mouse mAb against Jurkat (1) cell lysate.
- Flow cytometric analysis of Hela cells using NDC80 mouse mAb (green) and negative control (red).
- Flow cytometric analysis of HepG2 cells using NDC80 mouse mAb (green) and negative control (red).
- Flow cytometric analysis of Jurkat cells using NDC80 mouse mAb (green) and negative control (red).
- Flow cytometric analysis of K562 cells using NDC80 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using NDC80 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using NDC80 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using NDC80 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using NDC80 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#32242
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