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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using HSP70 mouse mAb against Hela (1), HepG2 (2),Hek293 (3),COS-7 (4),A549 (5) and Jurkat (6) cell lysate.
Immunofluorescence analysis of Hela cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Jurkat cells using HSP70 mouse mAb (green) and negative control (red).
Flow cytometric analysis of K562 cells using HSP70 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Raji cells using HSP70 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using HSP70 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using HSP70 mouse mAb with DAB staining.
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Mouse Monoclonal Antibody to HSP70
-
货号：
32244 -
别名：
RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52 -
应用：
WB,IHC,IF,FCM -
反应种属：
Human, Monkey -
抗体类型：
Primary antibody -
Swissprot：
P34932 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to HSP70
Description |
|---|
HSPA4 (Heat Shock Protein Family A (Hsp70) Member 4) is a Protein Coding gene. Diseases associated with HSPA4 include Vulvovaginitis and Babesiosis. Among its related pathways are Cellular response to heat stress and Mechanisms of CFTR activation by S-nitrosoglutathione (normal and CF). An important paralog of this gene is HSPA4L. |
References |
|---|
| 1.J Biol Chem.2020 Jun 12;295(24):8302-8324. 2.Biol Chem.2020 Oct 25;401(11):1233-1248. |
Specification |
|
|---|---|
| Aliases | RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52 |
| Entrez GeneID | 3308 |
| Swissprot | P34932 |
| clone | 2E4F10B11 |
| WB Predicted band size | 94.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Monkey |
| Immunogen | Purified recombinant fragment of human HSP70 (AA: 642-841) expressed in mammalian. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Western blot analysis using HSP70 mouse mAb against Hela (1), HepG2 (2),Hek293 (3),COS-7 (4),A549 (5) and Jurkat (6) cell lysate.
- Immunofluorescence analysis of Hela cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of Jurkat cells using HSP70 mouse mAb (green) and negative control (red).
- Flow cytometric analysis of K562 cells using HSP70 mouse mAb (green) and negative control (red).
- Flow cytometric analysis of Raji cells using HSP70 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using HSP70 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using HSP70 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#32244
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