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Western blot analysis using H2AFX mouse mAb against HEK293 (1), HepG2 (2), Jurkat (3),Hela (4), Raji (5), K562 (6) and HCT116 (7) cell lysate.
Immunofluorescence analysis of Hela cells using H2AFX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using H2AFX mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using H2AFX mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using H2AFX mouse mAb with DAB staining.

Mouse Monoclonal Antibody to H2AFX

  • 货号:

    32386
  • 别名:

    H2AX; H2A.X; H2A/X
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF,FCM
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P16104
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to H2AFX

Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

References

1.Radiat Res. 2021 Mar 1;195(3):244-252.
2.Mutat Res Genet Toxicol Environ Mutagen. 2020 Aug-Sep;856-857:503220.

Specification

Aliases H2AX; H2A.X; H2A/X
Entrez GeneID 3014
Swissprot P16104
clone 4A2D5
WB Predicted band size 15.1kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of human H2AFX expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IHC 1/200 - 1/1000
IF 1/50 - 1/200
FCM 1/200 - 1/400
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