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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using RPA1 mouse mAb against HeLa (1), MCF-7 (2), K562(3), A431(4), and COS-7 (6) cell lysate.
Immunofluorescence analysis of HeLa cells using RPA1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of Jurkat cells using RPA1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using RPA1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human prostate tissues using RPA1 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to RPA1
-
货号：
30348 -
别名：
HSSB; RF-A; RP-A; REPA1; RPA70; MST075 -
应用：
WB,IHC,IF,FCM -
反应种属：
Human,Monkey -
抗体类型：
Primary antibody -
Swissprot：
P27694 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to RPA1
Description |
|---|
"This gene Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing" |
References |
|---|
| Mol Cell. 2009 Oct 23;36(2):193-206. J Biol Chem. 2009 Dec 11;284(50):34682-91. |
Specification |
|
|---|---|
| Aliases | HSSB; RF-A; RP-A; REPA1; RPA70; MST075 |
| Entrez GeneID | 6117 |
| Swissprot | P27694 |
| clone | 4C4 |
| WB Predicted band size | 68.1kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | Purified recombinant fragment of human RPA1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using RPA1 mouse mAb against HeLa (1), MCF-7 (2), K562(3), A431(4), and COS-7 (6) cell lysate.
- Immunofluorescence analysis of HeLa cells using RPA1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow cytometric analysis of Jurkat cells using RPA1 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using RPA1 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human prostate tissues using RPA1 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30348
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