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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using PCNA mouse mAb against A431 (1), HEK293 (2), HeLa (3), HepG2 (4), Raji (5), MOLT4 (6), COS7 (7), and MCF-7 (8) cell lysate.
Western blot analysis using PCNA mouse mAb against Raw264.7(1)NIH/3T3(2)NRK(3)C2C12(4)C6(5)L1210(6)F9(7)CHO3D10(8) cell lysate.
Flow cytometric analysis of MOLT4 cells using PCNA mouse mAb (green) and negative control (purple).
Flow cytometric analysis of Hela cells using PCNA mouse mAb (green) and negative control (red).
Flow cytometric analysis of NIH/3T3 cells using PCNA mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using PCNA mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using PCNA mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Mouse colon(A) Mouse testis(B) using PCNA mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat testis using PCNA mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit testis(A) Rabbit kidney (B)using PCNA mouse mAb with DAB staining.

Mouse Monoclonal Antibody to PCNA

  • 货号:

    30453A
  • 别名:

  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,FCM
  • 反应种属:

    Human,Mouse,Rat,Rabbit,Monkey
  • 抗体类型:

    Loading Control Antibody
  • Swissprot:

    P12004
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1320

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Mouse Monoclonal Antibody to PCNA

Description

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

References

1.Cancer Res. 2012 Jul 1;72(13):3217-27.
2.PLoS One. 2012;7(1):e29416.

Specification

Entrez GeneID 5111
Swissprot P12004
clone 7H4F8
WB Predicted band size 28.7kDa
Host/Isotype Mouse IgG1
Antibody Type Loading Control Antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat,Rabbit,Monkey
Immunogen Purified recombinant fragment of human PCNA (AA: 53-196 ) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.

Application

WB 1/500 - 1/2000
IHC 1/100 - 1/500
FCM 1/200 - 1/400
ELISA 1/10000

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#30453A

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